The estranged wife, and two other men are facing second-degree murder charges in the death of U.S. Navy Cmdr. Alphonso Doss.
The Clay County Sheriff's Office arrested 44-year-old Yolinda Doss, 33-year-old Ronnie Wilson II, and 28-year-old Anthony Washington.
Deputies found the body of Cmdr. Doss in a room at the Astoria Hotel in Orange Park in February.
He had been strangled.
Investigators believe the trio devised a plan to kill Doss and make it look like a burglary. Authorities said Wilson and Washington carried out the attack while Yolinda Doss served as a lookout.
The motive was money, according to authorities. Doss served in the Navy since 1987 and was close to retirement.
"We believe their goal was to benefit financially from this crime," said Orange Park Police Chief Gary Goble. "We believe it was substantial in life insurance," he said. "Commander Doss was a Navy veteran and had life insurance policies."
Wilson and Doss lived together in Orange Park, according to the Clay County Sheriff's Office. Washington, of Jacksonville, worked with Wilson and was in a relationship with Doss.
The suspects are being held without bond at the Clay County Jail. They will be in court Wednesday morning.